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KMID : 0363020060360020335
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Journal of Korean Academy of Periodontology
2006 Volume.36 No. 2 p.335 ~ p.344
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Inhibition of mRANKL Expression by Doxycycline in Rat Periodontal Ligament Cells
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조관표
최득철/김영준
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Abstract
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Osteoblast or bone marrow stromal cell-derived RANKL is the major effector molecule essential for
osteoclastogenesis. Previous studies have shown that tetracyclines have beneficial therapeutic effects
in the prevention and treatment of inflammatory bone disease including periodontal disease.
Periodontal ligament cells are thought not only to play an important role in the progression of periodontal disease, but to play an important role in alveolar bone remodeling. Previous studies indicated that receptor activation of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) are
expressed in periodontal ligament cells by pro-inflammatory cytokine, such as IL-1β and TNF-α.
This study was designed to investigate the inhibitory effect of doxycycline on RANKL and OPG
mRNA in rat periodontal ligament cells induced by IL-1β(1 ng/ml).
The results are as follows;
1. MTT assay showed that doxycycline at the concentration of 1-50 ㎍/㎖ didn't result in statistically significant cell death at day 1 and 3 .
2. RANKL mRNA expression was increased to 2.6 folds by IL-1β. When cells were treated with
doxycycline (50 ㎍/㎖), IL-1β-induced mRANKL expression was reduced by 33%. In contrast to
RANKL, OPG mRNA expression was not inhibited by pre-treatment with doxycycline.
These results suggest that doxycycline decrease the expression of mRANKL resulting in regulation
of osteoclastogenesis in rat periodontal ligament cells.2)
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KEYWORD
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RT-PCR, doxycycline, mRANKL, mOPG, periodontal Ligament cell
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